Immunization Schedule:
Day -3,-1
One pre-immune egg/day from each bird
Day 1
First treatment, requires 0.5ml prepared Ag
Day 7
Second treatment, requires 0.5ml prepared Ag
Day 21-26
One egg/day from each bird immunized
Antigen Preparation:
Antigen needs to be at least 10-12 Dalton to elicit good antibody production. Necessary antigen quantities range from 200ug to 2mg per bird.
Antigen should be prepared in PBS. The concentration can range from 400ug/ml to 4mg/ml. A total of 1ml prepared antigen is needed per bird for both treatments.